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Literature summary extracted from

  • Itoh, T.; Ochiai, A.; Mikami, B.; Hashimoto, W.; Murata, K.
    A novel glycoside hydrolase family 105: the structure of family 105 unsaturated rhamnogalacturonyl hydrolase complexed with a disaccharide in comparison with family 88 enzyme complexed with the disaccharide (2006), J. Mol. Biol., 360, 573-585.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.172 expression in Escherichia coli Bacillus subtilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.172 hanging drop vapor diffusion method, crystal structure of YteR complexed with unsaturated chondroitin disaccharide (substrate of bacterial unsaturated glucuronyl hydrolase). The substrate is sterically hindered with the active pocket of YteR. The protruding loop of YteR prevents the UGL substrate from being bound effectively Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.172 0.1
-
 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose 30°C, pH 4.0 Bacillus subtilis
3.2.1.172 0.719
-
 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose 30°C, pH 6.0 Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.172 39000
-
 gel filtration Bacillus subtilis
3.2.1.172 43000
-
 gel filtration Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.172 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O Bacillus subtilis the enzyme is involved in the degradation of the rhamnogalacturonan I main chain in Bacillus subtilis 4-deoxy-alpha-L-threo-hex-4-enopyranuronate + L-rhamnopyranose
-
 ?
3.2.1.172 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O Bacillus subtilis 168 the enzyme is involved in the degradation of the rhamnogalacturonan I main chain in Bacillus subtilis 4-deoxy-alpha-L-threo-hex-4-enopyranuronate + L-rhamnopyranose
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.172 Bacillus subtilis O31521
-
-
3.2.1.172 Bacillus subtilis O34559
-
-
3.2.1.172 Bacillus subtilis 168 O31521
-
-
3.2.1.172 Bacillus subtilis 168 O34559
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.172 recombinant enzyme from Escherichia coli Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.172 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O
-
 Bacillus subtilis 4-deoxy-alpha-L-threo-hex-4-enopyranuronate + L-rhamnopyranose
-
 ?
3.2.1.172 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O the enzyme is involved in the degradation of the rhamnogalacturonan I main chain in Bacillus subtilis Bacillus subtilis 4-deoxy-alpha-L-threo-hex-4-enopyranuronate + L-rhamnopyranose
-
 ?
3.2.1.172 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O
-
 Bacillus subtilis 168 4-deoxy-alpha-L-threo-hex-4-enopyranuronate + L-rhamnopyranose
-
 ?
3.2.1.172 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O the enzyme is involved in the degradation of the rhamnogalacturonan I main chain in Bacillus subtilis Bacillus subtilis 168 4-deoxy-alpha-L-threo-hex-4-enopyranuronate + L-rhamnopyranose
-
 ?
3.2.1.172 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O
-
 Bacillus subtilis 4-deoxy-alpha-L-threo-hex-4-enopyranuronate + alpha-L-rhamnopyranose
-
 ?
3.2.1.172 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose + H2O
-
 Bacillus subtilis 168 4-deoxy-alpha-L-threo-hex-4-enopyranuronate + alpha-L-rhamnopyranose
-
 ?
3.2.1.172 additional information no activity with: DELTAGlcA-GalNAc, DELTAGlcA-GlcNAc, and DELTAGalA-(GalA)n Bacillus subtilis ?
-
 ?
3.2.1.172 additional information no activity with: DELTAGlcA-GalNAc, DELTAGlcA-GlcNAc, and DELTAGalA-(GalA)n Bacillus subtilis 168 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.172 monomer 1 * 43000, SDS-PAGE Bacillus subtilis
3.2.1.172 monomer 1 * 39000, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
3.2.1.172 YesR
-
 Bacillus subtilis
3.2.1.172 YteR
-
 Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.172 30
-
 assay at Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.172 50
-
 stable below Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.172 0.28
-
 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose 30°C, pH 4.0 Bacillus subtilis
3.2.1.172 13.9
-
 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose 30°C, pH 6.0 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.172 4
-
-
 Bacillus subtilis
3.2.1.172 6
-
-
 Bacillus subtilis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.172 4.8 7.2 pH 4.8: about 40% of maximal activity, pH 7.2: about 55% of maximal activity Bacillus subtilis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.172 2.8
-
 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose 30°C, pH 4.0 Bacillus subtilis
3.2.1.172 19.3
-
 2-O-(4-deoxy-beta-L-threo-hex-4-enopyranuronosyl)-alpha-L-rhamnopyranose 30°C, pH 6.0 Bacillus subtilis